Characterization of the Gene Encoding Serine Acetyltransferase, a Regulated Enzyme of Cysteine Biosynthesis from the Protist ParasitesEntamoeba histolyticaand …
T Nozaki, T Asai, LB Sanchez, S Kobayashi… - Journal of Biological …, 1999 - ASBMB
The enteric protist parasitesEntamoeba histolytica and Entamoeba disparpossess a cysteine
biosynthetic pathway, unlike their mammalian host, and are capable of de novo production
ofl-cysteine. We cloned and characterized cDNAs that encode the regulated enzyme serine
acetyltransferase (SAT) in this pathway from these amoebae by genetic complementation of
a cysteine-auxotrophic Escherichia coli strain with the amoebic cDNA libraries. The deduced
amino acid sequences of the amoebic SATs exhibited, within the most conserved region, 36 …
biosynthetic pathway, unlike their mammalian host, and are capable of de novo production
ofl-cysteine. We cloned and characterized cDNAs that encode the regulated enzyme serine
acetyltransferase (SAT) in this pathway from these amoebae by genetic complementation of
a cysteine-auxotrophic Escherichia coli strain with the amoebic cDNA libraries. The deduced
amino acid sequences of the amoebic SATs exhibited, within the most conserved region, 36 …
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