Continuation of a phytochemical study of Cichorium intybus L.(Astraceae) growing in Egypt, resulted in the isolation and identification of a new sesquiterpene lactone 3, 4-dihydrolactucin, in addition to the eight known compounds; kaempferol, isoscutellarin, cichoriin, umbelliferone, lupeol, lupeol acetate, , and . Chemical structures of the isolated compounds were assigned based on different physical, chemical, and spectroscopic techniques including IR, UV, MS, 1D-and 2D-NMR spectra. Furthermore, the antimicrobial, and spasmogenic activities of some fractions and isolates were also assessed.