[HTML][HTML] Clonal relation and antimicrobial resistance pattern of extended-spectrum β-lactamase- and AmpC β-lactamase-producing Enterobacter spp. isolated from …

R Ghanavati, M Emaneini… - Revista da Sociedade …, 2018 - SciELO Brasil
Revista da Sociedade Brasileira de Medicina Tropical, 2018SciELO Brasil
INTRODUCTION: Here, we determined the genes encoding antibiotic resistance enzymes
and virulence factors and evaluated the genetic relationship between Enterobacter spp.
isolated from different clinical samples. METHODS: A total of 57 clinical isolates of
Enterobacter spp. were tested for the production of extended-spectrum β-lactamases
(ESBLs), carbapenemase, and AmpC using phenotypic and genotypic methods. RESULTS:
The most common ESBLs and AmpC β-lactamases were bla TEM (63.3%) and bla EBC …
INTRODUCTION
Here, we determined the genes encoding antibiotic resistance enzymes and virulence factors and evaluated the genetic relationship between Enterobacter spp. isolated from different clinical samples.
METHODS
A total of 57 clinical isolates of Enterobacter spp. were tested for the production of extended-spectrum β-lactamases (ESBLs), carbapenemase, and AmpC using phenotypic and genotypic methods.
RESULTS
The most common ESBLs and AmpC β-lactamases were bla TEM (63.3%) and bla EBC (57.7%), respectively. The most prevalent virulence gene was rpos (87.7%). The random amplified polymorphic DNA (RAPD) patterns of strains were genetically unrelated.
CONCLUSIONS
RAPD polymerase chain reaction analysis revealed high genetic diversity among isolates.
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