V2O5/TiO2 is an important catalyst for the partial oxidation of o-xylene to phthalic anhydride. This selective oxidation is highly exothermic process and the yield of product strongly depends on the stability and activity of catalyst. Investigation on commercially available catalyst V2O5/TiO2 revealed that it deactivates at high temperature, which disrupt the Titania particles exposing the vanadium directly for reaction. This causes the decrease in shelf life of commercial catalyst. In this work, V2O5/TiO2 was prepared by sol-gel method and characterized by XRD, TGA, BET, EDS and SEM. XRD pattern revealed the presence of well crystalline V2O5/TiO2 while SEM showed the homogeneous nanoparticles morphology grown by controlled chemical reaction parameters of the sol-gel method. In this study, investigation and comparative analysis of morphology and elemental analysis has been conducted between commercially available and lab synthesized V2O5/TiO2 catalysts. This work revealed the importance of nanoscale catalyst due to its thermal stability and high surface area than commercially available catalyst.