Comparative evaluation of PCR success with universal primers of maturase K (matK) and ribulose-1, 5-bisphosphate carboxylase oxygenase large subunit (rbcL) for …

SO Bafeel, IA Arif, MA Bakir, HA Khan… - Plant Omics, 2011 - search.informit.org
DNA barcoding is the use of short DNA sequences (650 bp) of the standard segment of the
genome for large scale species identification. The Consortium for the Barcode of Life
(CBOL) plant-working group recommended the 2-locus combination of rbcL and matK as the
standard plant barcode. These two regions of chloroplast DNA were chosen due to efficient
recovery of quality sequences and high levels of species discrimination. We evaluated the
success rates of universal primers for amplification of matK and rbcL loci in 26 different plant …

[PDF][PDF] Comparative evaluation of PCR success with universal primers of maturase K (matK) and ribulose-1, 5-bisphosphate carboxylase...

DNA barcoding is the use of short DNA sequences (~ 650 bp) of the standard segment of the
genome for large scale species identification. The Consortium for the Barcode of Life
(CBOL) plant-working group recommended the 2-locus combination of rbcL and matK as the
standard plant barcode. These two regions of chloroplast DNA were chosen due to efficient
recovery of quality sequences and high levels of species discrimination. We evaluated the
success rates of universal primers for amplification of matK and rbcL loci in 26 different plant …
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