[PDF][PDF] Comparison of antigen detection methods of peste des petits ruminants virus in clinical samples of small ruminants
Bulgarian J Vet Med, 2011•academia.edu
Peste des petits ruminants (PPR) is an acute and highly contagious viral disease of small
ruminants such as goats and sheep. The diagnosis of PPR infection in sheep and goats
populations can be strengthened with detection of antigen in clinical samples of susceptible
populations. In present study, the PPR virus antigen detection was investigated and
compared in clinical sheep and goat samples by agar gel immunodiffusion (AGID),
haemagglutination (HA) tests and immuno-capture ELISA (IC-ELISA). The viral antigen was …
ruminants such as goats and sheep. The diagnosis of PPR infection in sheep and goats
populations can be strengthened with detection of antigen in clinical samples of susceptible
populations. In present study, the PPR virus antigen detection was investigated and
compared in clinical sheep and goat samples by agar gel immunodiffusion (AGID),
haemagglutination (HA) tests and immuno-capture ELISA (IC-ELISA). The viral antigen was …
Peste des petits ruminants (PPR) is an acute and highly contagious viral disease of small ruminants such as goats and sheep. The diagnosis of PPR infection in sheep and goats populations can be strengthened with detection of antigen in clinical samples of susceptible populations. In present study, the PPR virus antigen detection was investigated and compared in clinical sheep and goat samples by agar gel immunodiffusion (AGID), haemagglutination (HA) tests and immuno-capture ELISA (IC-ELISA). The viral antigen was detected from 8.95% of the samples tested by AGID, 20.9% tested by HA test and 34.3% tested by IC-ELISA. Kappa statistics scores for HA versus AGID, IC-ELISA versus AGID and HA versus IC-ELISA were 0.45 (95% confidence interval CI 0.158–0.742), 0.359 (95% CI 0.01–0.69) and 0.035 (95% CI 0.309–0.694), respectively, and indicated a non-significant agreement between the tests. The major findings of this study were that the HA test was more sensitive than AGID for detection of PPRV antigen, but IC-ELISA was the most sensitive of all three. The rapid detection of viral antigen by appropriate methods will help in early diagnosis of infection and subsequent control of the PPR disease in Pakistan.
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