… cube delivery (Fig. 33.5C). The relevance of these in vitro data to the in vivo recognition of …
of therapeutic oligonucleotides [53] and nucleic-acid-containing nanoparticles [54], we tested …

… for ex vivo delivery into cultured cell most likely will not correlate with optimal in vivo delivery
[24]. Reflecting all these hurdles, how can we optimize macromolecular drug delivery? Here …

… been approved as most successful representatives of ex vivo cancer gene therapeutics [3]. …
vivo cancer gene therapies. Early research with plasmid DNA (pDNA) focused on the delivery …

… by the nucleic acid-containing nanoparticles upon … are some common obstacles for in vivo
delivery of either siRNA or pDNA. … applications should have several components that function …

… strategies requires the in vivo delivery of active ODN or siRNA … high transfection efficiency,
these components are known to … PLGA around the nucleic acid containing inner water phase …

… in a broad distribution of nanoparticle sizes and compositions. Electrostatic interactions are
fairly … Nucleic acid-containing polymer nanoparticles are often prepared using two aqueous …

… of viral gene delivery systems when applied in vivo [14,15]. … of delivering nucleic acids due
to their lipid composition-… methodology for preparing nucleic acid-containing GUV liposomes …

… the lipid composition to achieve beneficial surface composition, … components, enable a
degree of targeted nucleic acid … corona composition, and ultimately the in vivo biodistribution …

… vivo due to their small size. One way to circumvent these problems is to conjoin PNAs with
nanoparticles … The availability of particles with tunable compositions and properties will allow …

… , and material composition, nanoparticles shape has also been … was developed from boronic
acid-containing block copolymers, … vesicles as protein carriers for in vivo delivery. Improved …