STUDY QUESTION Can simultaneous comprehensive chromosome screening (CCS) and
gene expression analysis be performed on the same biopsy of preimplantation human
embryos? SUMMARY ANSWER For the first time, CCS and reliable gene expression
analysis have been performed on the same human preimplantation embryo biopsy. WHAT
IS KNOWN ALREADY A single trophectoderm (TE) biopsy is routinely used for many IVF
programs offering CCS for selection of only chromosomally normal embryos for transfer …

Objective While comprehensive chromosome screening (CCS) has demonstrated
improvement in outcomes in 3 published randomized trials, many euploid embryos fail to
implant and progress to delivery illustrating the need for additional biomarkers of
reproductive potential. The transcriptome represents a provocative target for investigation.
However, methods which allow simultaneous assessment have not been established. This
study aims to develop and validate a new methodology in which both RNA and DNA can be …