In the present work, α-and β-amylase enzymes from Zea mays malt were recovered by
continuous extraction in a PEG/CaCl2 aqueous two-phase system (ATPS). The influences of
the flux rate (RQ), free area of vane (Afree) and vane rotation (RV) on enzyme recovery were
studied by optimization using response surface methodology (RSM). The protein content
and enzyme activity were measured from time to time in the extract and refined fluxes. RSM
curves showed a squared dependence of recovery index with the RQ, Afree and RV. The …