Resistance to fluconazole (FLC), the most widely used antifungal drug, is typically achieved
by altering the azole drug target and/or drug efflux pumps. Recent reports have suggested a
link between vesicular trafficking and antifungal resistance. Here, we identified novel
Cryptococcus neoformans regulators of extracellular vesicle (EV) biogenesis that impact
FLC resistance. In particular, the transcription factor Hap2 does not affect the expression of
the drug target or efflux pumps, yet it impacts the cellular sterol profile. Subinhibitory FLC …