Fast angiogenesis in 3D fibrous constructs that mimic the morphology of the extracellular
matrix remains challenging due to limited porosity in the densely packed constructs. We
investigated whether mimicking the in vivo chemotaxis microenvironment for native blood
vessel formation would stimulate angiogenesis in the fibrous constructs. The chemotaxis
microenvironment was created by introducing 3D angiogenic growth factor gradients into the
constructs. We have developed a technique that can quickly fabricate (∼ 40 min) such 3D …