Cryopreservation of kunming mouse oocytes using slow cooling, ultrarapid cooling and vitrification protocols
The cryopreservation of oocytes has been only marginally successful with any of the current
protocols, including slow cooling, rapid cooling and vitrification. We wished to test the
hypothesis that oocytes from a single mouse strain would freeze successfully by 1 of the 3
mentioned protocols. Unfertilized Kunming mouse oocytes obtained 14 h after PMSG/hCG
administration were randomly assigned to be cryopreserved after slow cooling, ultra rapid
cooling and vitrification. Oocytes were thawed by straws being placed into 37° C water, and …
protocols, including slow cooling, rapid cooling and vitrification. We wished to test the
hypothesis that oocytes from a single mouse strain would freeze successfully by 1 of the 3
mentioned protocols. Unfertilized Kunming mouse oocytes obtained 14 h after PMSG/hCG
administration were randomly assigned to be cryopreserved after slow cooling, ultra rapid
cooling and vitrification. Oocytes were thawed by straws being placed into 37° C water, and …
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