Cryopreservation of Shoot Tips of Elite Cultivars of Cannabis sativa L. by Droplet Vitrification
Cannabis sativa L.(marijuana or hemp) is recognized worldwide for its psychoactive
properties as well as for fiber production. This study focused on the evaluation of 3 droplet
vitrification protocols for long-term conservation of shoot tips in liquid nitrogen (LN). Shoot
tips (∼ 0.5 mm) were excised from 3-to 4-week-old in vitro-grown shoots of 3 cultivars (MX,
VI-20, and B-5: high tetrahydrocannabinol [THC], high cannabidiol [CBD], and intermediate
THC∼ CBD, respectively) and pretreated on 5% dimethyl sulfoxide agar plates for 48 h. The …
properties as well as for fiber production. This study focused on the evaluation of 3 droplet
vitrification protocols for long-term conservation of shoot tips in liquid nitrogen (LN). Shoot
tips (∼ 0.5 mm) were excised from 3-to 4-week-old in vitro-grown shoots of 3 cultivars (MX,
VI-20, and B-5: high tetrahydrocannabinol [THC], high cannabidiol [CBD], and intermediate
THC∼ CBD, respectively) and pretreated on 5% dimethyl sulfoxide agar plates for 48 h. The …
[PDF][PDF] Cryopreservation of Shoot Tips of Elite Cultivars of Cannabis sativa L. by Droplet Vitrification
MA ElSohlya - 2019 - academia.edu
Cannabis sativa L.(marijuana or hemp) is recognized worldwide for its psychoactive
properties as well as for fiber production. This study focused on the evaluation of 3 droplet
vitrification protocols for long-term conservation of shoot tips in liquid nitrogen (LN). Shoot
tips (∼ 0.5 mm) were excised from 3-to 4-week-old in vitro-grown shoots of 3 cultivars (MX,
VI-20, and B-5: high tetrahydrocannabinol [THC], high cannabidiol [CBD], and intermediate
THC∼ CBD, respectively) and pretreated on 5% dimethyl sulfoxide agar plates for 48 h. The …
properties as well as for fiber production. This study focused on the evaluation of 3 droplet
vitrification protocols for long-term conservation of shoot tips in liquid nitrogen (LN). Shoot
tips (∼ 0.5 mm) were excised from 3-to 4-week-old in vitro-grown shoots of 3 cultivars (MX,
VI-20, and B-5: high tetrahydrocannabinol [THC], high cannabidiol [CBD], and intermediate
THC∼ CBD, respectively) and pretreated on 5% dimethyl sulfoxide agar plates for 48 h. The …
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