… embryonic stem cells and induced pluripotent stem cells, are … cell survival and rapid
proliferation and that culture systems must thus provide. Current 2D-based cell culture systems—…

… culture system suitable for the expansion of pluripotent ESCs. mESCs were used as our
initial model, allowing for extensive optimization of seeding and culture … more rapid mESC …

… 3-D culture system for propagation and maintenance of mouse ESCs utilizing Dex-SH and
PEG-4-Acr. Cells … with the lower cell concentration (2 × 10 6 cells/mL) resulted in rapid clonal …

… Hence, a defined culture system with known composition of culture medium is desirable to
… Standardized and optimized culture systems are needed, and the normality of the cells has …

… study contained serum, resulting in rapid differentiation of HES cells. … A culture system using
human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells. …

… , methods for hPSC culture have evolved rapidly in an attempt … Despite rapid progress in
developing new hPSC culture methods, … substrate that supports human embryonic stem cell self-…

… expand multipotent, self-renewing pre-rosette neural stem cells from both human embryonic
stem cells … simple protocol allows rapid expansion of early multipotent stem cells that carry a …

… Since then, rapid progress has been achieved and numerous … systems, isolation of ICM
cells using human antibodies or mechanical methods, growth of hES cells in culture systems …

… Over the past two decades, human embryonic stem cells (hESCs) … the limitation of feeder
cell-dependent culture systems, mainly owing … Removal of Activin A on the contrary led to rapid …

… a scalable culture system for hiPSC expansion using the E8 … stem cells (hiPSCs) and
human embryonic stem cells (hESCs… approach for rapid, economic, and scalable expansion of …