We have recently shown that the physiological mediator of granule-mediated apoptosis is a
macromolecular complex of granzymes and perforin complexed with the chondroitin-sulfate
proteoglycan, serglycin (Metkar, SS, Wang, B., Aguilar-Santelises, M., Raja, SM, Uhlin-
Hansen, L., Podack, E., Trapani, JA, and Froelich, CJ (2002) Immunity 16, 417–428). We
now report our biophysical studies establishing the nature of granzyme B-serglycin (GrB·
SG) complex. Dynamic laser light scattering studies establish that SG has a hydrodynamic …