Detection of contaminants in cell cultures, sera and trypsin
TFP de Oliveira, AA Fonseca Jr, MF Camargos… - Biologicals, 2013 - Elsevier
The aim of this study was standardization and application of polymerase chain reaction
(PCR) for the detection of contaminants in cell cultures, sera and trypsin. Five PCR protocols
were standardized to assess the presence of genetic material from mycoplasma, porcine
circovirus 1 (PCV1), bovine leukemia virus (BLV) or bovine viral diarrhea virus (BVDV) in
cell culture samples. PCR reactions for the genes GAPDH and beta-actin were used to
evaluate the efficiency of nucleic acid extraction. The PCR protocols were applied to 88 cell …
(PCR) for the detection of contaminants in cell cultures, sera and trypsin. Five PCR protocols
were standardized to assess the presence of genetic material from mycoplasma, porcine
circovirus 1 (PCV1), bovine leukemia virus (BLV) or bovine viral diarrhea virus (BVDV) in
cell culture samples. PCR reactions for the genes GAPDH and beta-actin were used to
evaluate the efficiency of nucleic acid extraction. The PCR protocols were applied to 88 cell …
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