Simple Summary Anhydrobiosis is a unique ametabolic state that is characterized by
extreme body water loss due to desiccation, generally over 95%. The sleeping chironomid,
Polypedilum vanderplanki, is an anhydrobiotic insect inhabiting semi-arid regions in Africa.
Pv11 is an anhydrobiotic insect cell line established from the P. vanderplanki larvae, and the
cell line also displays the extreme desiccation tolerance. This unique feature could be useful
for developing a dry preservation technique. However, effective expression systems for …

… Here we report the identification of an effective minimal promoter for Pv11 cells and its
application to the Tet-On inducible expression system. First, using a luciferase reporter
assay, we showed that a 202 bp deletion fragment derived from the constitutively active 121-promoter
functions in Pv11 cells as an appropriate minimal promoter with the Tet-On inducible
expression system. The AcGFP1 (Aequorea coerulescens green fluorescent protein) was
also successfully expressed in Pv11 cells using the inducible system. In addition to these …