Differential sensitivity of low molecular weight DNA polymerase to sulfhydryl-blocking reagents
A Matsukage, EW Bohn, SH Wilson - … Acta (BBA)-Nucleic Acids and Protein …, 1975 - Elsevier
Activity of a 2.5 S mouse myeloma DNA polymerase (termed DNA polymerase II) measured
with either poly (rA) or poly (dA) as template did not require sulfhydryl-reducing reagents, but
was sensitive to inhibition by p-hydroxymercuribenzoate and the sulfhydryl-alkylating
reagent, N-ethylmaleimide; however, the activity was much more sensitive to inhibition by p-
hydroxymercuribenzoate than by the sulfhydryl-alkylating reagent. The p-
hydroxymercuribenzoate inhibition appeared to involve the mercurial portion of the p …
with either poly (rA) or poly (dA) as template did not require sulfhydryl-reducing reagents, but
was sensitive to inhibition by p-hydroxymercuribenzoate and the sulfhydryl-alkylating
reagent, N-ethylmaleimide; however, the activity was much more sensitive to inhibition by p-
hydroxymercuribenzoate than by the sulfhydryl-alkylating reagent. The p-
hydroxymercuribenzoate inhibition appeared to involve the mercurial portion of the p …
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