In vivo functional imaging at single-neuron resolution is an important approach to visualize
biological processes in neuroscience. Light sheet microscopy (LSM) is a cutting edge in vivo
imaging technique that provides micron-scale spatial resolution at high frame rate. Due to
the scattering and absorption of tissue, however, conventional LSM is inadequate to resolve
cells because of the attenuated signal to noise ratio (SNR). Using dual-beam illumination
and confocal dual-slit detection, here a dual-slit confocal LSM is demonstrated to obtain the …

… Abstract: We developed a dual-slit confocal light sheet microscopy which enhanced the
image SNR and obtained two-fold imaging rate compared with conventional line scanning
confocal microscopy. … In conclusion, the dual-slit confocal light sheet microscopy actually
enhanced SNR and contrast than the conventional LSM with the experiments by 200nm
fluorescent beads and in vivo larval zebrafish imaging. Through the rolling shutter exposure
mode by the sCMOS, we achieved two-fold imaging rate for the conventional confocal line …