… The 16S protocol detailed here is designed to amplify prokaryotes (bacteria and archaea)
using paired-end 16S community sequencing on the Illumina platform. Primers 515F-806R …

… of several commonly used protocols for extracting bacterial DNA from ants. While bacterial
community composition recovered using Illumina 16S rRNA amplicon sequencing was not …

… Illumina sequencing was performed on the MiSeq platform following the manufacturer’s
guidelines… The 356,661 sequences represented 203 amplicon sequence variants (ASVs). The …

… of the 16S rRNA gene and containing Illumina flow cell adaptors and a single index
sequence. (b) The DI protocol used in this study, in which the V4 variable region of the 16S rRNA …

… protocol that we describe in this study adds a third Illumina … a two-stage PCR protocol (as
recommended by Illumina [6]), … In addition, our protocol includes the heterogeneity spacers …

… miniaturized amplicon library preparation protocol and demonstrate its utility using the EMP
16S … Since it is common to multiplex 768 samples on a single Illumina sequencing lane, we …

… (NGS) using the 16S rRNA gene (16S rDNA) has been widely … of total Illumina reads
retrieved from PCR amplicons using … with our terms or guidelines please flag it as inappropriate. …

… (EMP) 16S rRNA gene amplicon profiling protocol [1, 3] for quantitative microbiota profiling.
This protocol … Long “overhangs” (carrying the linker and Illumina adapter sequences) at the 5…

… a detailed standardized protocol of high-throughput 16S rRNA-amplicon sequencing. The
protocol introduces an integrated, uniformed, feasible, and inexpensive protocol starting from …

… a stepwise protocol to characterize the diversity of eukaryotic microbes in a sample, using
… or proceed to) Illumina library generation. A protocol for 16S sequencing library preparation …