Advances in high-resolution cryo-electron microscopy (cryo-EM) require the development of
validation metrics to independently assess map quality and model geometry. We report
EMRinger, a tool that assesses the precise fitting of an atomic model into the map during
refinement and shows how radiation damage alters scattering from negatively charged
amino acids. EMRinger (https://github. com/fraser-lab/EMRinger) will be useful for
monitoring progress in resolving and modeling high-resolution features in cryo-EM.