Sludge composting is increasingly adopted due to its end product for application as a soil nourishment amendment. Although the ratio of C/N is significant in the quality and process of composting, little information has been obtained from the effects of nutritional energy (carbon and nitrogen) on the fate of antibiotic resistance genes (ARGs) during sludge composting. Dynamic variations of ARGs, microbial community as well as functional characteristics during composting of sludge were investigated in this study. Three levels of carbon to nitrogen (20:1, 25:1 and 30:1) were developed for the composting of sludge with fermented straw plus a control which was just sewage sludge (C/N = 9.5:1). A novel finding of this work is that the highest initial C/N ratio (30:1) could prolong the thermophilic period, which was helpful to reduce some target ARGs. Some ARGs (sul1, sul2, and aadA1) had negative correlation with multiple metabolic pathways, which were difficult to remove.