The aim of the present work was to study the effect of ammonia and lactate on growth,
metabolism, and productivity of BHK cells producing a recombinant fusion protein. Results
show that cell growth was reduced with the increase in ammonia or lactate: k1/2 of 1.1 mM
and 3.5 mM for stirred and stationary cultures, respectively, for ammonia and of 28 mM for
both stationary and stirred cultures for lactate, were obtained. The cell-specific consumption
rates of both glucose (qGlc) and glutamine (qGln) increased, whereas that of oxygen (qO2) …