[HTML][HTML] Effects of hesperidin as a radio-protector on apoptosis in rat peripheral blood lymphocytes after gamma radiation
R Fardid, Z Ghorbani, G Haddadi… - Journal of biomedical …, 2016 - ncbi.nlm.nih.gov
Journal of biomedical physics & engineering, 2016•ncbi.nlm.nih.gov
Materials and Methods: 64 male rats were divided into eight groups: Control, HES (100
mg/kg bw, orally, 7 days), whole body irradiation with 2 and 8Gy, pre-administrated with 50
and 100 mg/kg body weight of Hesperidin for 7 days before irradiation with 2 and 8 Gy. 24
hours after radiation, apoptotic lymphocytes were evaluated using PE Annexin V Apoptosis
detection I kit and the levels of mRNA for bax and bcl-2 were evaluated by real time reverse
transcription polymerase chain reaction. Results: A significant reduction in apoptosis of the …
mg/kg bw, orally, 7 days), whole body irradiation with 2 and 8Gy, pre-administrated with 50
and 100 mg/kg body weight of Hesperidin for 7 days before irradiation with 2 and 8 Gy. 24
hours after radiation, apoptotic lymphocytes were evaluated using PE Annexin V Apoptosis
detection I kit and the levels of mRNA for bax and bcl-2 were evaluated by real time reverse
transcription polymerase chain reaction. Results: A significant reduction in apoptosis of the …
Materials and Methods:
64 male rats were divided into eight groups: Control, HES (100 mg/kg bw, orally, 7 days), whole body irradiation with 2 and 8Gy, pre-administrated with 50 and 100 mg/kg body weight of Hesperidin for 7 days before irradiation with 2 and 8 Gy. 24 hours after radiation, apoptotic lymphocytes were evaluated using PE Annexin V Apoptosis detection I kit and the levels of mRNA for bax and bcl-2 were evaluated by real time reverse transcription polymerase chain reaction.
Results:
A significant reduction in apoptosis of the lymphocytes was demonstrated in group animals receiving 8 Gy compared to the group which received 2 Gy irradiation (p< 0.0001). However, apoptosis significantly increased in group of rats who received Hesp before irradiation (p< 0.05). The increase of apoptosis by Hesperidin administration can be attributed to the decreased expression of bax and significantly reduced expression of bcl-2 and finally increasing the ration of bax/bcl-2.
Conclusion:
The results suggest that administration of 50 and 100 mg/kg of Hesperidin induces apoptotic effects by changing expression level of bax, bcl-2 and also the ratio of bax/bcl2.
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