[HTML][HTML] Eicosanoid content in fetal calf serum accounts for reproducibility challenges in cell culture
L Niederstaetter, B Neuditschko, J Brunmair, L Janker… - Biomolecules, 2021 - mdpi.com
Reproducibility issues regarding in vitro cell culture experiments are related to genetic
fluctuations and batch-wise variations of biological materials such as fetal calf serum (FCS).
Genome sequencing may control the former, while the latter may remain unrecognized.
Using a U937 macrophage model for cell differentiation and inflammation, we investigated
whether the formation of effector molecules was dependent on the FCS batch used for
cultivation. High resolution mass spectrometry (HRMS) was used to identify FCS …
fluctuations and batch-wise variations of biological materials such as fetal calf serum (FCS).
Genome sequencing may control the former, while the latter may remain unrecognized.
Using a U937 macrophage model for cell differentiation and inflammation, we investigated
whether the formation of effector molecules was dependent on the FCS batch used for
cultivation. High resolution mass spectrometry (HRMS) was used to identify FCS …
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