Energy and protein sources for development of pig embryos cultured beyond hatching in vitro
BA Stone, P Quinn, RF Seamark - Animal Reproduction Science, 1984 - Elsevier
The effects of supplementation of synthetic culture media with different energy and protein
sources on in vitro development of pig embryos beyond the 4–8-cell stage have been
explored. Minimal Essential Medium (MEM) supplemented with glucose (1 mg/ml) proved
superior to Krebs-Ringer Bicarbonate (KRB) supplemented with glucose (1 mg/ml) in its
capacity to support embryonic development to the expanded blastocyst stage (P< 0.05).
Inclusion of pyruvate (0.25 mM) or lactate (25 mM) in either MEM or KRB based media …
sources on in vitro development of pig embryos beyond the 4–8-cell stage have been
explored. Minimal Essential Medium (MEM) supplemented with glucose (1 mg/ml) proved
superior to Krebs-Ringer Bicarbonate (KRB) supplemented with glucose (1 mg/ml) in its
capacity to support embryonic development to the expanded blastocyst stage (P< 0.05).
Inclusion of pyruvate (0.25 mM) or lactate (25 mM) in either MEM or KRB based media …
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