Next-generation sequencing (NGS) has been extensively employed to perform microbiome
characterization worldwide. As a culture-independent methodology, it has allowed high-
level profiling of sample microbial composition. However, most studies are limited to
information regarding relative bacterial abundances, ignoring scenarios in which sample
microbe biomass can vary widely. Here, we develop an equivolumetric protocol for amplicon
library preparation capable of generating NGS data responsive to input DNA, recovering …

High-throughput sequencing of 16S rRNA amplicon has been extensively employed to
perform microbiome characterization worldwide. As a culture-independent methodology, it
has allowed high-level profiling of sample bacterial composition directly from samples.
However, most studies are limited to information regarding relative bacterial abundances
(sample proportions), ignoring scenarios in which sample microbe biomass can vary widely.
Here, we use an equivolumetric protocol for 16S rRNA amplicon library preparation capable …