Due to the challenges brought by field measurements to estimate the aboveground biomass (AGB), such as the remote locations and difficulties in walking in these areas, more accurate and cost-effective methods are required, by the use of remote sensing. In this study, Sentinel-2 data were used for estimating the AGB in pure stands of Carpinus betulus (L., common hornbeam) located in the Hyrcanian forests, northern Iran. For this purpose, the diameter at breast height (DBH) of all trees thicker than 7.5 cm was measured in 55 square plots (45 × 45 m). In situ AGB was estimated using a local volume table and the specific density of wood. To estimate the AGB from remotely sensed data, parametric and nonparametric methods, including Multiple Regression (MR), Artificial Neural Network (ANN), k-Nearest Neighbor (kNN), and Random Forest (RF), were applied to a single image of the Sentinel-2, having as a reference the estimations produced by in situ measurements and their corresponding spectral values of the original spectral (B2, B3, B4, B5, B6, B7, B8, B8a, B11, and B12) and derived synthetic (IPVI, IRECI, GEMI, GNDVI, NDVI, DVI, PSSRA, and RVI) bands. Band 6 located in the red-edge region (0.740 nm) showed the highest correlation with AGB (r = −0.723). A comparison of the machine learning methods indicated that the ANN algorithm returned the best ABG-estimating performance (%RMSE = 19.9). This study demonstrates that simple vegetation indices extracted from Sentinel-2 multispectral imagery can provide good results in the AGB estimation of C. betulus trees of the Hyrcanian forests. The approach used in this study may be extended to similar areas located in temperate forests.