Thrombus and embolus formation may partially or totally obstruct blood flow. This may lead life threatening cardiovascular and cerebrovascular disorders. In obsessive conditions; thrombolytic operators are utilized to disintegrate the effectively shaped clusters in the veins in any case, these medications have certain restrictions which cause genuine and some of the time lethal results. Herbal preparations have been used since ancient times for the treatment of several diseases. Herbal drugs possess variety of phytoconstituents. These phytoconstituents are prominent and promising pharmacological agents with good safety profile. The aim of this study was to identify safe and effective thrombolytic agent from herbal origin, in-vitro. An in-vitro thrombolytic model was utilized to check the coagulation lysis impact of methanolic concentrate of Prunus avium L. organic product alongside Streptokinase as a positive control and DMSO as a negative control. Using an in-vitro thrombolytic model, Prunus avium L. different concentration (5μg/ml, 10μg/ml, 25μg/ml, and 50μg/ml) showed 25.6%, 32.5%, 54.6%, and 59.7% clot lysis activity respectively. Although% clot lysis was found higher with all concentration of extract, the statistically significant increase was found with 25μg/ml and 50μg/ml extract with reference to DMSO as negative control (10.6%) Streptokinase (88.7%) as standard. These herbal extracts possess thrombolytic properties that could lyse blood clots in vitro; however, further assessment of in-vivo thrombolytic activity is necessary to explore its therapeutics benefits.