Expression, purification, and functional characterization of an N-terminal fragment of the tomato mosaic virus resistance protein Tm-1
M Kato, K Ishibashi, C Kobayashi, M Ishikawa… - Protein expression and …, 2013 - Elsevier
Tm-1, the protein product of Tm-1, a semidominant resistance gene of tomato, inhibits
tomato mosaic virus (ToMV) replication by binding to ToMV replication proteins. Previous
studies suggested the importance of the Tm-1 N-terminal region for its inhibitory activity;
however, it has not been determined if the N-terminal region is sufficient for inhibition.
Furthermore, the three-dimensional structure of Tm-1 has not been determined. In this study,
an N-terminal fragment of Tm-1 (residues 1–431) as a fusion protein containing an upstream …
tomato mosaic virus (ToMV) replication by binding to ToMV replication proteins. Previous
studies suggested the importance of the Tm-1 N-terminal region for its inhibitory activity;
however, it has not been determined if the N-terminal region is sufficient for inhibition.
Furthermore, the three-dimensional structure of Tm-1 has not been determined. In this study,
an N-terminal fragment of Tm-1 (residues 1–431) as a fusion protein containing an upstream …
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