Expression of an 8R-lipoxygenase from the coral Plexaura homomalla
NC Gilbert, DB Neau, ME Newcomer - Methods in enzymology, 2018 - Elsevier
Methods are presented for the use of the coral 8R-lipoxygenase from the Caribbean sea
whip coral Plexaura homomalla as a model enzyme for structural studies of animal
lipoxygenases. The 8R-lipoxygenase is remarkably stable and can be stored at 4° C for 3
months with virtually no loss of activity. In addition, an engineered “pseudo wild-type”
enzyme is soluble in the absence of detergents, which helps facilitate the preparation of
enzyme: substrate complexes.
whip coral Plexaura homomalla as a model enzyme for structural studies of animal
lipoxygenases. The 8R-lipoxygenase is remarkably stable and can be stored at 4° C for 3
months with virtually no loss of activity. In addition, an engineered “pseudo wild-type”
enzyme is soluble in the absence of detergents, which helps facilitate the preparation of
enzyme: substrate complexes.
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