Among the previously established enzyme-linked immunosorbent assays (ELISAs), an
ELISA using the full length of a recombinant thrombospondin-related adhesive protein of
Babesia gibsoni (rBgTRAPf) is considered as the most sensitive diagnostic method for the
detection of an antibody to B. gibsoni in dogs. However, the expression of rBgTRAPf in high
concentration is poor and, thus, limits its usefulness as a diagnostic antigen. To improve its
expression level, we have truncated BgTRAPf into two fragments having either an N-or a C …