Extending the limits of quantitative proteome profiling with data-independent acquisition and application to acetaminophen-treated three-dimensional liver …
The data-independent acquisition (DIA) approach has recently been introduced as a novel
mass spectrometric method that promises to combine the high content aspect of shotgun
proteomics with the reproducibility and precision of selected reaction monitoring. Here, we
evaluate, whether SWATH-MS type DIA effectively translates into a better protein profiling as
compared with the established shotgun proteomics. We implemented a novel DIA method
on the widely used Orbitrap platform and used retention-time-normalized (iRT) spectral …
mass spectrometric method that promises to combine the high content aspect of shotgun
proteomics with the reproducibility and precision of selected reaction monitoring. Here, we
evaluate, whether SWATH-MS type DIA effectively translates into a better protein profiling as
compared with the established shotgun proteomics. We implemented a novel DIA method
on the widely used Orbitrap platform and used retention-time-normalized (iRT) spectral …
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