Extraction and three-phase partitioning behavior of proteases from papaya peels
Dried papaya peels exhibited superior proteolytic activity to the fresh peels. An extraction
with phosphate buffer pH 7.0 greatly maintained proteolytic activity when compared to water.
SDS-PAGE verified that the extracted dried papaya peels held a wide range of proteins. To
optimize the three-phase partitioning (TPP) for isolating the papaya peel proteases required
a ratio of crude extract to t-butanol, the (NH4) 2SO4 concentration and the TPP cycles. The
ratio of the crude extract to t-butanol of 1.0: 0.5 with the presence of 20%(NH4) 2SO4 …
with phosphate buffer pH 7.0 greatly maintained proteolytic activity when compared to water.
SDS-PAGE verified that the extracted dried papaya peels held a wide range of proteins. To
optimize the three-phase partitioning (TPP) for isolating the papaya peel proteases required
a ratio of crude extract to t-butanol, the (NH4) 2SO4 concentration and the TPP cycles. The
ratio of the crude extract to t-butanol of 1.0: 0.5 with the presence of 20%(NH4) 2SO4 …
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