Failure of the IDA in FRET Systems at Close Inter-Dye Distances Is Moderated by Frequent Low κ2 Values
JD Spiegel, S Fulle, M Kleinschmidt… - The Journal of …, 2016 - ACS Publications
Förster resonance energy transfer (FRET) is analyzed in terms of distance-and orientation-
dependent interactions between the transition dipole moments of the involved donor and
acceptor molecules. However, the ideal dipole approximation (IDA) is known to fail at short
donor–acceptor distances. In this work, we model FRET in a Cy5-and Alexa Fluor 488-
labeled double-stranded RNA by means of combined molecular dynamics (MD) simulations
and quantum-chemical calculations involving the IDA as well as the more sophisticated …
dependent interactions between the transition dipole moments of the involved donor and
acceptor molecules. However, the ideal dipole approximation (IDA) is known to fail at short
donor–acceptor distances. In this work, we model FRET in a Cy5-and Alexa Fluor 488-
labeled double-stranded RNA by means of combined molecular dynamics (MD) simulations
and quantum-chemical calculations involving the IDA as well as the more sophisticated …
[引用][C] Failure of the IDA in FRET Systems at Close Inter-Dye Distances Is Moderated by Frequent Low κ2 Values
SJ Dominik, F Simone, K Martin, G Holger - 2016
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