High throughput sequencing technologies have enhanced our knowledge of variations in genetic sequences. Consequently, breeding programs around the world are incorporating genomic assisted selection into their projects. PCR applications relevant to plant breeding often depend on small quantities of DNA suitable for quick diagnostic tests for a relatively large number of samples. The method presented here is a modification of existing protocols. We eliminated heating incubations and omitted usage of potentially noxious chemicals. DNA extraction from wheat, barley, and triticale grains was conducted and the utility of extracted wheat DNA was tested by using molecular markers for a stem rust resistance gene (Sr26), photoperiod sensitivity locus (Ppd-D1), and adult plant resistance gene block (Yr18-Lr34).