The presented materials deal with the topical problem of rhizogenesis induction and rooting of genus Paulownia plants obtained by in vitro method. According to the research results, the optimal composition of the nutrient medium for the regenerants cultivation on the stage of rhizogenesis induction has been defined, the technologically optimal age of the regenerants for the in vitro - ex vitro transition, the depth of the seedlings, the number of post-septic nipples has been established. The obtained results allow to improve the technological process at the stages of rhizogenesis induction and plants rooting during the post-septic adaptation.