Objective: Fetal membranes, a vital component that helps maintain pregnancy and
contribute to parturition signaling, are often studied in segments due to its structural
complexity. Transwells are traditionally used to study cell interactions; however, their
usefulness is limited. To overcome these difficulties, a fetal membrane-organ-on-chip (FM-
OO-C) was created to study interactive properties of amnion epithelial cells (AECs) and
decidual cells compared to transwell systems. Methods: Primary AECs and decidual cells …

The amnion membrane that lines the human intrauterine cavity is composed of amnion
epithelial cells (AECs) connected to an extracellular matrix containing amnion mesenchymal
cells (AMCs) through a basement membrane. Cellular interactions and transitions are
mechanisms that facilitate membrane remodeling to maintain its integrity. Dysregulation of
cellular remodeling, primarily mediated by oxidative stress (OS), is often associated with
preterm birth. However, the mechanisms that maintain membrane homeostasis remain …