Fluorescent Leishmania species: development of stable GFP expression and its application for in vitro and in vivo studies
Reporter genes have proved to be an excellent tool for studying disease progression.
Recently, the green fluorescent protein (GFP) ability to quantitatively monitor gene
expression has been demonstrated in different organisms. This report describes the use of
Leishmania tarentolae (L. tarentolae) expression system (LEXSY) for high and stable levels
of GFP production in different Leishmania species including L. tarentolae, L. major and L.
infantum. The DNA expression cassette (pLEXSY-EGFP) was integrated into the …
Recently, the green fluorescent protein (GFP) ability to quantitatively monitor gene
expression has been demonstrated in different organisms. This report describes the use of
Leishmania tarentolae (L. tarentolae) expression system (LEXSY) for high and stable levels
of GFP production in different Leishmania species including L. tarentolae, L. major and L.
infantum. The DNA expression cassette (pLEXSY-EGFP) was integrated into the …
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