The aim of the current research work was to formulate, optimize and assess micronized Silver sulfadiazine (mSSD) topical gel containing Aloe vera gel (AV-gel) for the healing effect on second degree burn wounds induced in Albino Wistar rats. Concentration of Carbopol 940, gelling agent, was selected based on observation of optimum spreadability and consistency. Second-degree burn wounds were developed on the posterior part of Albino Wistar rats under anaesthetic condition. The developed wounds were cured by application of mSSD gel once a day (one application every day) for 14 days post wounding, and observation was made until healing. The observation showed a comparison in the rate of wound healing between mSSD gel treated (Group II) and control group (Group I). The mSSD gel treated group showed higher extent of tissue hyperplasia as compared to control group. mSSD topical gel containing AV-gel treated animals showed, 84.96% healing up to 14 days, while control group animals showed 49.48% healing of wounds on 14th day. Inflammation and pus formation were not observed in treated animals, while control group showed evidence of inflammation and pus formation in the first week. In conclusion, prepared mSSD gel containing AV-gel shows potential outcome in the course of treatment of second degree burn wound through reversal of wound retarding process.