Gelatin embedding: a novel way to preserve biological samples for terahertz imaging and spectroscopy
Physics in Medicine & Biology, 2015•iopscience.iop.org
Sample dehydration has traditionally been a challenging problem in ex vivo terahertz
biomedical experiments as water content changes significantly affect the terahertz properties
and can diminish important contrast features. In this paper, we propose a novel method to
prevent sample dehydration using gelatin embedding. By looking at terahertz image data
and calculating the optical properties of the gelatin-embedded sample, we find that our
method successfully preserves the sample for at least 35 h, both for imaging and …
biomedical experiments as water content changes significantly affect the terahertz properties
and can diminish important contrast features. In this paper, we propose a novel method to
prevent sample dehydration using gelatin embedding. By looking at terahertz image data
and calculating the optical properties of the gelatin-embedded sample, we find that our
method successfully preserves the sample for at least 35 h, both for imaging and …
Abstract
Sample dehydration has traditionally been a challenging problem in ex vivo terahertz biomedical experiments as water content changes significantly affect the terahertz properties and can diminish important contrast features. In this paper, we propose a novel method to prevent sample dehydration using gelatin embedding. By looking at terahertz image data and calculating the optical properties of the gelatin-embedded sample, we find that our method successfully preserves the sample for at least 35 h, both for imaging and spectroscopy. Our novel preservation method demonstrates for the first time the capability to simultaneously maintain sample structural integrity and prevent dehydration at room temperature. This is particularly relevant for terahertz studies of freshly excised tissues but could be beneficial for other imaging and spectroscopy techniques.
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