Online learning (OL) has become an important part of university education. In the past OL was applied in different universities with different forms. With rapid technological developments OL gains a new format through Internet. Students can take courses online wherever they are geographically. It provides working people and adults possibility to enroll in a higher education institution without time and space barriers. Beginning from 1980s, Turkey adapted its education system to this new form through open education faculties in state universities. However as the number of foundation universities increase, OL became more common application in many programs at different levels. In this study, the effect of genders of students who enrolled in an online program on service quality perception in Turkey will be determined and analyzed through statistical analyses.