The AmpF ‘STR® IdentifilerTM kit has recently been accepted for use in DNA databasing of forensic samples in the FBI’s National DNA Index System. In the present study, we used this kit to analyze the allele distribution of 15 short tandem repeat markers (STR) in individuals living in Caracas city, Venezuela. The allele frequencies of two of these STR, D2S1338 and D19S433, have not previously been reported for this or any other Latin American population. The results indicate that for the population here studied, the 15 STR tested are useful markers for paternity testing and forensic casework.© 2003 Elsevier Ireland Ltd. All rights reserved.