Background Cotton is one of the most important commercial crops as the source of natural
fiber, oil and fodder. To protect it from harmful pest populations number of newer transgenic
lines have been developed. For quick expression checks in successful agriculture qPCR
(quantitative polymerase chain reaction) have become extremely popular. The selection of
appropriate reference genes plays a critical role in the outcome of such experiments as the
method quantifies expression of the target gene in comparison with the reference …

Background: With the advent of newer breeds and transgenic varieties of commercial crops,
qPCR (quantitative polymerase chain reaction) experiments have become extremely
popular for quick expression checks. Selection of appropriate reference genes plays a
critical role in quantifying the expression of target gene. Most commonly used reference
genes in expression studies are the “house-keeping genes”, involved in basic cellular
processes. However, expression levels of such genes often vary in response to …