The contribution of Mycobacterium bovis to the global burden of tuberculosis (TB) in man is
likely to be underestimated due to its dysgonic growth characteristics and because of the
absence of pyruvate in most used media is disadvantageous for its primary isolation. In
Brazil Mycobacterium culture, identification and susceptibility tests are performed only in TB
reference centers, usually for selected cases. Moreover, solid, egg-based, glycerol-
containing (without pyruvate supplementation) Löwenstein-Jensen (LJ) or Ogawa media are …