Textile industry is evolving towards limiting the use of synthetic dyes due to their environmental and health concerns. Natural dyes are considered the ultimate alternative as it is green and ecofriendly. However, natural dyeing is limited by the use of metal mordants to enhance the colorimetric and fastness behavior of dyed textiles. In this work, madder roots (Rubia Tinctorum) from Morocco were analyzed by mean of HPLC-PDA and used to dye wool yarns. The effect of pre-mordanting with different metal mordants was assessed. Color properties, exhaustion, and fixation rates of dyed wool was also evaluated depending on the mordants.