In the present study, the effect of various carbon sources such as glucose, fructose and sucrose on growth kinetics and ethanol productivity of Saccharomyces cerevisiae strain PTCC 24860 was investigated. The growth kinetic parameters were determined with Malthus, Monod and Logistic rate equations. The computed maximum specific growth rates by Monod kinetic model were highest. Then Monod model was used to fit the growth kinetic data. The obtained maximum specific growth rates, µ_max and the Monod constants, K_s, for various substrates of glucose, fructose and sucrose were 0.65, 1.35, 1.85 h^-1 and 11.39, 39.19, 97.82 g/l, respectively. Fermentation with media contained fructose; a maximum ethanol concentration of 14.45 g/l was obtained. The maximum ethanol productivity using fructose was 1.22 and 1.38-folds higher than glucose and sucrose, respectively.