… careful application of the Eisen and Smith guidelines [3], MO-… set of guidelines regarding
the use of MOs in zebrafish that we … These general guidelines build upon those by Eisen and …

… Here we summarize screening with zebrafish (Danio rerio) as a model organism using
antisense morpholino oligonucleotides (MOs) as the primary gene-specific knockdown approach. …

… accordance with relevant institutional and national guidelines and regulations and were
approved by the North Carolina State University Institutional Animal Care and Use Committee. …

… Several groups have used antisense morpholino oligonucleotides in an effort to characterise
the role of tmem88a in zebrafish cardiovascular development, but they have not obtained …

… in ZF, these genes can be knocked down by injecting morpholinos into early stage embryos.
Validation of the morpholino needs to be performed and the concern of aspecific effects can …

… Morpholino antisense … Morpholinos in the zebrafish serve as a powerful reverse genetics
tool by knocking down gene function. In this article, we will microinject a morpholino oligo …

… zebrafish 32 . Thus, several recommendations and guidelines for morpholino use in zebrafish
… it is recommended that experimenters follow these guidelines. In our view, when possible, …

… off both in the entire zebrafish embryo and in single cells. We use these tools to demonstrate
… in compliance with guidelines of the University of Oregon Animal Care and Use Committee. …

We read with great interest the commentary by Li and Uitto (2018) on our recent article
describing a mutant, a morpholino (MO), and a CRISPR/Cas9-based zebrafish model of …

… This study aimed to clarify the role of Atoh8 in zebrafish development, using a genetic
mutant not subject to the pitfalls of morpholino artefacts. No overt abnormalities were noted in …