This study assesses the impact of hunting on the densities of nonhuman primates in two indigenous Tikuna territories (Mocagua and San Martín), overlapping Amacayacu National Park in the Colombian Amazon. Large‐bodied primates were once favored prey by Tikunas, but are now rarely hunted owing to the diminishing primate populations. We evaluate the effect of a hunting ban on woolly monkeys (Lagothrix lagothricha) by the residents of Mocagua, using qualitative and quantitative methods. Hunting records showed that from February 2005 to February 2009, a total of 25,142 kg of mammal bushmeat were harvested in Mocagua and San Martín. Primates constituted 345 kg of the total harvest. From 223 kg of large‐bodied primates extracted for subsistence purposes, 160 kg were hunted in San Martín and 64 kg in Mocagua. Large‐bodied primates made up 70% of the total primate biomass in Mocagua (398 kg/km²) and 22% in San Martín (199 kg/km²). From dietary records, we found bushmeat constituted 30% of protein consumption in Mocagua and 37% in San Martín. Primates were absent in records from Mocagua, and appeared only three times in those from San Martín suggesting inconsistencies with hunting data. Despite its moderate consumption, bushmeat was identified as a highly valued food source during focus group activities. Primate pet‐keeping and part utilization were observed in San Martín but not in Mocagua, possibly as a consequence of fewer primates being hunted. We suggest that Mocagua provides an example of how community‐based conservation strategies can be achieved, where opportunities for employment in tourism and alternative food sources are available. Am. J. Primatol. 72:855–865, 2010. © 2010 Wiley‐Liss, Inc.