Methods

A total of 375 blood-donating Saudi men were recruited from the Central Blood Bank in Madinah, the Kingdom of Saudi Arabia (KSA), and stratified into three age groups: 18-30, 31-40, and 41-61 years. Anti-HCMV IgG and IgM antibody levels were measured by enzyme-linked immunosorbent assay (ELISA). Pearson’s correlation coefficient was used to correlate antibody levels with variables.

Results

Most of the tested samples (95.73%, n= 356) were positive for anti-HCMV IgG antibodies, but only 1.6%(n= 6) were positive for both IgM and IgG antibodies, and all of them belonged to the age groups of 31-40 and 41-61 years. A strong inverse correlation was found between anti-HCMV IgG antibody levels and age (r=− 0.51, p< 0.0001). Additionally, there was an inverse correlation between anti-HCMV IgG antibody levels and body mass index (BMI)(r=− 0.11, p= 0.036). No correlations were found between anti-HCMV IgG levels and hemoglobin levels or blood groups of the participants.

Conclusions

Blood-donating Saudi men in Madinah had a high seroprevalence of anti-HCMV IgG antibodies, indicating previous viral exposure. Age and BMI might influence the humoral immunologic memory response against HCMV, which appears to be endemic in Madinah.